Journal: NPG Asia Materials

Article Title: Spatiotemporal regulation of endogenous MSCs using a functional injectable hydrogel system for cartilage regeneration

doi: 10.1038/s41427-021-00339-3

Figure Lengend Snippet: Fig. 10 MSC homing in cartilage defects at 7 days after the operation. A Immunofluorescent staining for CD44 and CD90. B, C Quantitative results for CD44- and CD90-positive cells.

Article Snippet: At 1 week postoperatively, samples were assessed by immunofluorescence staining for CD44 (1:100, NBP222530F, Novus) and CD90 (1:100, ab225, Abcam) to identify endogenous MSC recruitment.

Techniques: Staining

Journal: Cancers

Article Title: Overexpression of Cystine/Glutamate Antiporter xCT Correlates with Nutrient Flexibility and ZEB1 Expression in Highly Clonogenic Glioblastoma Stem-like Cells (GSCs)

doi: 10.3390/cancers13236001

Figure Lengend Snippet: High Gln/Glu ratios predict ZEB1 and xCT expression in primary GBM tumors. ( a ) Expanded regions from 1 H-NMR spectra showing Gln and Glu multiplets of metabolic extracts from seven primary glioblastoma (pGBM) patient samples. Spectra were normalized to the Glu content to visualize the differences in Gln/Glu ratios. ( b ) Immunoblotting of ZEB1, xCT, CD133, CD44, and cMYC protein in GBM patient samples arranged according to their Gln/Glu ratios in ascending order (loading control = β actin). A non-neoplastic brain sample of a trauma patient was blotted as a control. Abbreviations: ctrl, control; Gln, glutamine; Glu, glutamate; n.d., not detectable; pGBM, primary glioblastoma; ppm, parts per million. correspond to b.

Article Snippet: We applied the following primary antibodies and used the indicated dilutions: ZEB1 (1:2000, Sigma, #HPA027524), CD133 (1:250, Miltenyi Biotec, Bergisch Gladbach, Germany, #W6B3C1), c-Myc (1:1000, Thermo Fisher Scientific, #9E10), SOX2 (1:1000, Cell Signaling Technology, Cambridge, UK, #L1D6A2), xCT (1:1000, Thermo Fisher Scientific, #PA1-16893 and 1:1000, Cell Signaling Technologies, #12691), CD44 (1:2000, R&D Systems, Minneapolis, MN, USA, #AF3660), and β-actin (Thermo Fisher Scientific, #MA5-15739 and Cell Signaling Technology, #4970) were incubated overnight at 4 °C in 5% milk powder in Tris-buffered saline + 0.1% Tween-20 (TBST).

Techniques: Expressing, Western Blot, Control

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 1: Cytoplasmic and nuclear staining of HIF1α, CD133, and CD44 antibodies. HIF1α (A-F); A: Strong nuclear staining, B: Moderate nuclear staining, C: Weak nuclear staining, D: Strong cytoplasmic staining, E: Moderate cytoplasmic staining, F: Weak cytoplasmic staining. CD133 (G-M); G: Strong cytoplasmic staining, H: Moderate cytoplasmic staining, I: Weak cytoplasmic staining, K: Strong nuclear staining, L: Moderate nuclear staining, M: Weak nuclear staining. CD44 (N-P); N: Strong cytoplasmic staining, O: Moderate cytoplasmic staining, P: Weak cytoplasmic staining.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques: Staining

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 2: Mean H scores for cytoplasmic and nuclear staining of HIF1α, CD133, CD44 antibodies and comparative with grade.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques: Staining

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 3: Comperative distribution of cases according to H score threshold values (HIF1α nuclear, CD133 cytoplasmic- nuclear, CD44 cytoplasmic) and grades.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques:

Journal: Turkish Neurosurgery

Article Title: The relationship between the hypoxic process and cancer stem cells in meningioma

doi: 10.5137/1019-5149.jtn.27546-19.2

Figure Lengend Snippet: Figure 5: Distribution of cases with high HIF1α nuclear H scores according to the H score threshold values of CD133 cytoplasmic- nuclear and CD44 cytoplasmic staining.

Article Snippet: An immunohistochemical study was performed with HIF1α polyclonal antibody (1:200; Elabscience, USA), CD44 monoclonal antibody (1:500; Elabscience, USA), and CD133 polyclonal antibody (1:200; Elabscience, USA) using Ventana’s BenchMark Ultra automated system.

Techniques: Staining

Journal: Materials Today Bio

Article Title: A bioactive composite scaffold enhances osteochondral repair by using thermosensitive chitosan hydrogel and endothelial lineage cell-derived chondrogenic cell

doi: 10.1016/j.mtbio.2024.101174

Figure Lengend Snippet: EndMT-derived tEPCs acquire an MSC-like phenotype. ( a ) Flow cytometry analysis of CD44, CD90, CD105 (mesenchymal stem cell markers), CD34 (hematopoietic and endothelial cell marker), and CD45 (leukocyte marker) expression in tEPCs. The empty areas show isotype control staining. The red-filled areas represent the expression of specific markers. ( b ) Representative immunofluorescence images of tEPC surface markers. tEPCs stain positive for MSC markers (CD44, CD90, and CD105) and negative for endothelial cell markers (CD31, eNOS, VE-cadherin, and vWF) (20 × magnification; scale bar: 50 μm). ( c , d ) CFU efficiency of EPCs and tEPCs, assessing self-renewal through the rate of colony formation in CFU assays. ( c ) Representative colonies of EPCs and tEPCs in 6-well plates (scale bar: 5 mm). ( d ) Columns illustrate the CFU efficiency. Values are reported as the mean ± standard deviation (SD) of six replicates. *** P < 0.001. ( e ) Multilineage differentiation potential of tEPCs induced to differentiate into ( i ) chondrogenic (10 × magnification; scale bar: 100 μm), ( ii ) osteogenic (10 × magnification; scale bar: 100 μm), or ( iii ) adipogenic (40 × magnification; scale bar: 10 μm) lineages. Abbreviations: CD, cluster of differentiation; CFU, colony-forming unit; EndMT, endothelial-to-mesenchymal transition; eNOS, endothelial nitric oxide synthase; EPCs, endothelial progenitor cells; MSCs, mesenchymal stem cells; tEPC, transdifferentiated EPCs; VE-cadherin, vascular endothelial cadherin; vWF, von Willebrand factor.

Article Snippet: Next, 1 × 10 6 tEPCs were suspended in 500 μL of PBS containing 20 μg/mL of fluorescein isothiocyanate (FITC)-conjugated antibodies against CD44 (Novus, Centennial, CO, USA), CD90 (Bioworld, Louis Park, MN, USA), CD105 (Bioss, Woburn, MA, USA), CD34 (Bioss), and CD45 (Bioss).

Techniques: Derivative Assay, Flow Cytometry, Marker, Expressing, Control, Staining, Immunofluorescence, Standard Deviation